Legionella

Legionella bacteria, the cause of Legionnaire’s disease (a deadly pneumonia), aren’t just an historical problem—cases are on the rise in the US and around the world.

HotelFrom Science News, Sept. 7, 2015: This summer, in New York City’s worst ever outbreak of Legionnaires’ disease, left 12 dead and at least 100 infected. In two different outbreaks in an Illinois town in recent days at least 50 people have been diagnosed with the disease and 8 reported dead. Also in a California prison, 95 inmates are being watched, being suspected of being infected and six inmates were already confirmed to have contracted the disease.

The Centers for Disease Control (CDC), Occupational Safety & Health Administration (OSHA), and World Health Organization (WHO) have guidelines for detection, prevention, remediation, and treatment of Legionella. The release of ANSI/ASHRAE Standard 188-2015 and VHA Directive 1061 provide guidance for establishing a Water Management Program.

Abraxis is pleased to distribute Legipid® from Biotica, the only Legionella rapid detection system that quantitatively measures viable Legionella in water samples (from cooling towers, evaporative condensers, spas, pools, showers, sprinkler systems, surface water, etc.). It is an Immunomagnetic Separation Capture Enzyme Immunoassay (IMS-CEIA) format that is easy to use and AOAC certified. Legipid is a screening kit for fast, accurate, quantitative results that allow rapid action while awaiting confirmation by the much slower standardized culture methods (e.g. ISO 11731:1998). For more information, see References below.

Legipid Advantages:

Fast: Up to 40 tests in 1 hour (after filtering)
Selective for most (90%) Legionella spp. (unlike dipstick tests), including L. pneumophila
Sensitive, equal to or better than culturing
Visual or Optical (429 nm) Colorimetric Detection
Quantitative, results easily converted to cfu
Detects VBNC (Viable But Not Culturable) Legionella and biocide-treated samples
Easy to Multiplex with other water test analyses
Simple Workflow
High Value: unlike other methods, no complicated/expensive equipment necessary
Few False Negatives (unlike culture and PCR)
Few False Positives (unlike PCR, which also detects dead cells and free DNA)
AOAC Certified

Reference: Comparison of Legionella Detection Methods
White Paper: Monitoring Legionella to Address a Growing Problem

Legipid Workflow:
● Filter 1 liter water sample
● Add Legipid magnetic beads
● Wash and add antibody
● Add detection reagent
● Visual or Optical (429 nm) colorimetric detection

Video: How Legipid Works

Video: Legipid procedure with MP Hunter 4

Legipid Visual Detection

Visual Detection

Legipid Optical Detection

Optical Detection

Legionella Products

Legipid Legionella DetectionLegipid® Legionella IMS-CEIA Kits  Flyer  Brochure  Technical data
Provides reagents and vials to perform Legipid tests; magnetic holders available separately (see below). Fitration/concentration systems available separately (see below).
PN 311-10-01 (10 tests) User Guide
PN 311-10-02 (40 tests) User Guide
PN 311-10-04 (100 tests) User Guide

Legipid Magnetic Holders
MP Hunter 4
PN 311-MP4-SP Magnetic holder assembly, holds 4 test vials
PN 311-MP4-RA-M Holds 20 test vials (5 holder assemblies, 1 tablecloth, 1 platform & cover included)

Filtration/Concentration Systems
Filterpid Fast Filtration Kit
Easy, complete system (using 60 ml syringe) to prepare Legionella water samples for Legipid or Cleanpid.
PN 211-10-00 User Guide
Manual pump filtration system
PN 311-10-SFM-FP
Electric pump filtration system  Assembly
PN 311-10-SFC-FP User Guide

Cleanpid® Legionella Easy Purification Kit for PCR  Flyer
Simple and rapid method for the separation and purification of Legionella spp. in potable, natural, and industrial water. The method is based on magnetic bead immunocapture of Legionella cells after pre-concentration of samples (see filtration/concentration systems above).
PN 611-10-00 (50 tests) User Guide
PN 611-MRC-10 Magnetic holder for 10 tubes

Other Products
Primelab Photometer  Flyer
Absorbance/optical reader (380 nm – 780 nm) suitable for Legipid and other water tests, includes cuvette adaptor and 100 microcuvettes.
PN 911-10-PL
AllinOne portable lab-in-a-case  Flyer
Portable lab-in-a-case for on-site Legionella detection; includes magnetic holder, electric pump filtration system, and Primelab photometer. Purchase Legipid reagents separately (see kits above).
PN 411-10-AIO
Orbital Shaker
PN 706000 For use with Legipid MP Hunter 4 Magnetic holder assemblies
Vial, Round, Glass
For use with Legipid kit
PN 311-10-CB (quantity 1)

Legipid Legionella Pack 10


References:

AOAC study and independent lab validation: Method Modification of the Legipid® Legionella Fast Detection Test Kit: Performance Tested Method 111101. Yi Chen. Journal of AOAC International (2014) 97: 1403-1409. http://dx.doi.org/10.5740/jaoacint.14-029.

Comparison with culture method: Fast immunosensing technique to detect Legionella pneumophila in different natural and anthropogenic environments: comparative and collaborative trials. Begoña Bedrina, Sonia Macián, Inmaculada Solís, Roberto Fernández-Lafuente, Eva Baldrich and Guillermo Rodríguez. BMC Microbiology (2013) 13: 88. http://dx.doi.org/10.1186/1471-2180-13-88.

Independent comparison with culture and PCR methods: Comparing methods of determining Legionella spp. in complex water matrices. Álvaro Díaz-Flores, Juan Carlos Montero, Francisco Javier Castro, Eva María Alejandres, Carmen Bayón, Inmaculada Solís, Roberto Fernández-Lafuente and Guillermo Rodríguez. BMC Microbiology (2015) 15: 91. http://dx.doi.org/10.1186/s12866-015-0423-7.

Recent example of spread via cooling towers: Prevalence and Molecular Characteristics of Waterborne Pathogen Legionella in Industrial Cooling Tower Environments. Lijie Li, Tian Qin, Yun Li, Haijian Zhou, Hongmei Song, Hongyu Ren, Liping Li, Yongguang Li, and Dong Zhao. International Journal of Environmental Research and Public Health (2015) 12: 12605–12617. http://dx.doi.org/10.3390/ijerph121012605.

Recent example of spread via water fountains: An outbreak of Legionnaires disease associated with a decorative water wall fountain in a hospital. Thomas E. Haupta, Richard T. Heffernan, James J. Kazmierczak, Henry Nehls-Lowe, Bruce Rheineck, Christine Powell, Kathryn K. Leonhardt, Amit S. Chitnis and Jeffrey P. Davis. Infection Control & Hospital Epidemiology (2012) 33: 185-191. http://dx.doi.org/10.1086/663711