Algal Toxins

cyanotoxins testingAbraxis provides innovative, value added, comprehensive, real time, field and lab based testing solutions for both fresh and marine water algal toxins. Anatoxin-a, BMAA, Cylindrospermopsin, Microcystins and Saxitoxins test kits are the core for fresh water cyanotoxins testing; three of them are listed in the EPA CCL4. The multiple technologies, formats and applications available from Abraxis allow individuals, utilities, regional and research programs to easily achieve objectives.

As cyanotoxins testing expands into coastal waters, marine water applications are available for Cylindrospermopsin, Microcystins and Saxitoxins test kits. Additional applications for microcystins testing are available for bivalve and fish tissues as well as human serum. Brevetoxin (NSP), Domoic Acid (ASP), Okadaic Acid (DSP) and Saxitoxins (PSP) test kits are the core for the marine/shellfish poisons test kits. Shipboard/dockside testing applications are available for Brevetoxin, Domoic Acid, Okadaic Acid and Saxitoxins. An alternate technology, PP1A, is available for Okadaic Acid.

Contact us for protocols, and to discuss the method that best suits your needs.

Abraxis provides Innovative, Value Added, Comprehensive, Real time, Field and Lab Based Algal Toxin Testing Solutions for Cyanotoxins (fresh water) and Dinoflagellate toxins (shellfish poisoning):

  • ELISA, PP2A, and RBA Kits (Microtiter Plates, Tubes, and Strips)
  • Lysing and Filtering Alternatives
  • Microcystins Proficiency Testing Programs
  • Analytical Standards
  • Tri-Level Proficiency Testing Samples
  • Bivalve, Fish, Seawater, and Serum Applications
  • Readers, Automated Systems, System Installation
  • On-site Training and Technical Support

Fresh Water (Cyanotoxins)

Algae blooms are occurring more often, and more extensively, each year, in fresh water such as the Great Lakes, and marine water such as the Pacific coast. To see examples of state and local agency advisories and brochures, click on the following links: Advisory, MN Poster, OH Poster, OH Fact Sheet.

Before you get started, did you know that the type of container you use for sample collection could alter, or even ruin, your results? Check out our Cyanotoxin Sample Collection Guide

Assay Systems

Cyanotoxin Automated Assay System (CAAS) Image
PN 475200, Microtiter Plate, 96 well, without computer and printer, installation included.
*Video: Automated quantitation of cyanotoxins by ELISA using the Abraxis Cyanotoxin Automated Assay System (CAAS)
CAAS Training is also available; contact us for more details. Testimonial

Cyanotoxin Non-automated Assay System Alternatives

Fresh Water Kits

Anatoxin-a, ELISA Kit
Assay range 0.15 to 5.0 ppb (ug/L). The simplified protocol is similar to Cylindrospermopsin and Saxitoxin, with room temperature incubation and no sample concentration necessary. Samples should be immediately preserved with a solution provided in the kit, and can be used with both Anatoxin-a and Saxitoxin kits.
PN 520060, Microtiter Plate (96T) User Guide Flow Chart

Anatoxin-a, RBA Kit
PN 520050, Microtiter Plate (96T) User Guide Flow Chart Enhanced Sensitivity application NMS poster

BMAA, ELISA Kit
PN 520040, Microtiter Plate (96T) User Guide Flow Chart

Cylindrospermopsin, ELISA Kit
PN 522011, Microtiter Plate (96T) User Guide Flow Chart

Microcystins
We offer numerous Microcystin kit types and formats, each with its own strengths and advantages based on sample type, purpose of assay, etc. Contact us to discuss which kit best suits your needs. Protocols for sample collection, treatment, preparation, storage, and transportation of drinking and recreational water are available upon request.

Microcystins, ELISA Kit (tube format)
PN 520012A, Coated Tube (40T) User Guide Flow Chart
PN 472285, Rack, Separation, 6 positions Image
PN 530040, Accessory Pack, Lysing, Freeze-Thaw (20T)
PN 530038, Accessory Pack, Filtering (20T)

Microcystins/Nodularins (ADDA), ELISA Kit
PN 520011, Microtiter Plate (96T) User Guide Flow Chart EPA ETV Verification Estuary samples
PN 520011OH, Microtiter Plate (96T) User Guide Flow Chart EPA ETV Verification

Microcystins/Nodularins (ADDA) ES (Enhanced Sensitivity), ELISA Kit
PN 520011ES, Microtiter Plate (96T) Flow Chart Application

Microcystins/Nodularins-DM, ELISA Kit
PN 522015, Microtiter Plate (96T) User Guide Flow Chart EPA ETV Verification Estuary samples

Microcystins/Nodularins, PP2A Kits
PN 520032, Microtiter Plate (96T) User Guide Flow Chart
PN 520033, Tube format (32T) User Guide

Microcystins (Serum), ELISA Kit
PN 522031, Microtiter Plate (96T) User Guide Flow Chart Sample Prep Poster

QuikLyseTM Option* sample lysing and filtration in a matter of minutes! * patent pending
The Abraxis QuikLyse system is a sample preparation protocol for the rapid lysis of algal samples prior to ELISA testing. Lysis is necessary to measure total microcystins (dissolved, or free, plus cell bound). QuickLyse is not only faster, but unlike other lysis methods does not create potentially dangerous aerosols.
PN 529911 (45T) User Guide Flow Chart

Microcystins LR, Check Samples
Provides Microcystin LR standards to confirm accuracy, precision, and specificity of sample dilution and analysis.
PN 422011 User Guide

Saxitoxins, ELISA Kit
PN 52255B, Microtiter Plate (96T) User Guide Flow Chart Fresh Water Sample Prep

Proficiency Testing Programs – 2016

PN 2016-01, Drinking Water – Microcystins, March 2016 Sign-up
PN 2016-02, Drinking Water – Anatoxin-a and/or Cylindrospermopsin, March 2016 Sign-up
PN 2016-03, Recreational Water – Microcystins, June 2016

Fresh Water Strip Tests

Easy-to-use strip tests (or ‘dipsticks’) detect cyanotoxins: if the sample contains toxin over the level set by WHO (World Health Organization), the test will detect them even if there are no visible algal cells in the sample. The tests include internal controls and can be used as field tests–without laboratory equipment. Microcystin strip tests are available for drinking water (source and finished) and recreational water. Protocols for sample collection, treatment, preparation, storage and transportation are available upon request.
Flyer

1 ppb, Source Drinking Water with QuikLyseTM Option (up to 5 ppb)
PN 520019 (5T) User Guide Flow Chart
PN 520020 (20T) User Guide Flow Chart

1 ppb, Finished Drinking Water (up to 5 ppb)
PN 520016 (5T) User Guide Flow Chart
PN 520017 (20T) User Guide Flow Chart

10 ppb, Recreational Water with QuikLyseTM Option
PN 520023 (5T) User Guide Flow Chart EPA ETV Verification IL Program ’13 Beach Monitoring
PN 520022 (20T) User Guide Flow Chart EPA ETV Verification IL Program ’13 Beach Monitoring

AbraScan Test Strip Reader – Digital Reading of Dipsticks Simplifies Interpretation

Marine Water (Dinoflagellates & Diatoms / Shellfish Poisoning)

Brevetoxin (NSP), ELISA Kit
PN 520026, Microtiter Plate (96T) User Guide Flow Chart

Cyclic Imines (PnTX-A, PnTX-G, SPX-C), RBA Kit
PN 520060, Microtiter Plate (96T) User Guide

Domoic Acid (ASP), ELISA Kit (AOAC Method 2006.02)
PN 520501, Microtiter Plate (96T) User Guide
PN ON0021, Microtiter Plate (96T), Onsite User Guide

Okadaic Acid (DSP), ELISA Kit (EC 2002/225 Compliant)
PN 520021, Microtiter Plate (96T) User Guide Flow Chart

Okadaic Acid (DSP), PP2A Kit
PN 520025, Microtiter Plate (96T) User Guide Flow Chart

Saxitoxins (PSP), ELISA Kit (EC 2002/225 Compliant)
PN 52255B, Microtiter Plate (96T) User Guide Flow Chart
PN 52255SW, Seawater Standards

Saxitoxins (PSP) Shipboard, ELISA Kit (ISSC Certified, 09-107)
PN 52255SB, ELISA Kit Abstract FDA article
PN 530009, Accessory Pack
PN 470061C, Starter Pack with Computer

Analytical Standards

ANTXASTD
Anatoxin-a Fumarate Standard, 1.0 mg

BMAAD3STD-1.0
BMAA D3 Standard, 1.0 mg

CYLINDROSTD-0.5
Cylindrospermopsin Standard, 0.5 mg

CYLINDROSTD-S
Cylindrospermopsin Standard, 10 µg/ml, 1 ml

MICROLASTD-S
Microcystin LA Standard, 10 µg/ml, 1 ml

MICROLRSTD-0.5
Microcystin LR Standard, 0.5 mg

MICROLRSTD-S
Microcystin LR Standard, 10 µg/ml, 1ml

MICROLYSTD-S
Microcystin LY Standard, 10 µg/ml, 1ml

MICRORRSTD-S
Microcystin RR Standard, 10 µg/ml, 1 ml

MICROYRSTD-S
Microcystin YR Standard, 10 µg/ml, 1 ml

NODULARSTD-S
Nodularins Standard, 10 µg/ml, 1 ml

STXSTD
Saxitoxin Standard, 10 ug/mL in ethanol, 1 mL

STXSTDNL Technical info
Saxitoxin PSP N-15 Labeled Standard, 5 ug/mL in ethanol, 0.5 mL

Publications