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Veterinary Residues |
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Beta-Agonist, ELISA kit |
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PN 5061A, Microtiter Plate (96T) |
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Chloramphenicol, ELISA kit |
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PN 5091C, Microtiter Plate (96T)
| Chloramphenicol is a broad spectrum antibiotic which was widely used in veterinary medicine. Since 1994 the use of chloramphenicol is banned within the European Community. The Chloramphenicol EIA test is a competitive enzyme immunoassay for the screening of chloramphenicol in tissue, plasma, serum, urine, eggs, milk, honey and aqua culture products. Since the antibody used in this testsystem recognizes chloramphenicol-glucuronide, this substance can be analysed without prior hydrolysis. The dose-response curve shows a measuring range of 0.025-2 ng/ml. Diluted urine and defatted milk samples can be applied directly in the test with a detection limit of 0.1 ng/ml. In milk, a 10 times lower detection limit can be obtained by applying a simple extraction procedure. For all matrices extraction procedures are described in detection limits up to 0.01 ng/g. |
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Clenbuterol, ELISA kit |
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PN 5071C, Microtiter Plate (96T)
| This test is based on clenbuterol antibodies and it is recommended for the detection of the following Beta-Agonists (% of cross-reactivity): clenbuterol (100%), bromobuterol (100%), mapenterol (80%), mabuterol (70%) and cimbuterol (60%). The dose-response curve shows a measuring range for clenbuterol of 0.1 - 5 ng/ml or 5-250 pg/well. Urine can be used directly in this test after pH adjustment or, to lower background, after an extraction. For faeces, cattle feed, tissue, plasma, bile and choroid/ retina samples, simple extraction methods are described in the manual. Detection limits, depending on the Beta-Agonist, sample material and clean-up procedure, down to 0.05 ng/g can be reached. |
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Corticosteroids, ELISA kit |
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PN 5081C, Microtiter Plate (96T)
| Synthetic corticosteroids are widely used in livestock breeding to treat inflammatory diseases. MRLs for Dexamethasone in milk, tissue/kidney and liver are 0.3, 0.5 and 2.5 ng/kg respectively. Corticosteroids are found in cattle feed and are suspected to be used as growth-promoters. In all European Community countries however, the use of growth promoters has been banned since 1986. The generic Corticosteroid-EIA is a competitive enzyme immunoassay for the screening of feed, milk and urine on the presence of the following Corticosteroids (% of cross-reactivity): Flumethasone (125%), Dexamethasone (100%), Betamethasone (93%), Triamcinolone (33%) and Prednisolone (21). The dose-response curve shows a measuring range for Dexamethasone of 0.125-4 ng/ml or 6-200 pg/well. Milk can be used directly in this test with a sensitivity down to 0.15 ng/ml. For cattle feed samples, extraction methods are described in the manual and sensitivities down to 20 ng/g can be obtained. Urine samples can be applied after a dilution in buffer or after an HPLC fractionation. Tissue samples need an three phase extraction and clean up procedure. |
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Diethylstilbestrol, ELISA kit |
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PN 5081D, Microtiter Plate (96T)
| Diethylstilbestrol (DES), together with dienestrol and hexestrol belongs to the group of stilbenes. Increased weight gain and improved feed conversion after administration of DES has been observed in cattle, chicken and lambs. However, in the EC the use of anabolics has been banned, and especially DES has been banned in all countries due to its carcinogenic properties. The DES EIA can be used for the screening of urine samples on the presence of the following stilbenes (% of cross-reactivity): Diethylstilbestrol (100%), Hexestrol (25%) and Dienestrol (15%). The DES EIA shows no cross-reactivity (< 0.1%) for Zeranol, 19- Nortestosterone, Progesterone, Cortisol and Testosterone. Detection limits down to 0.2 ng/ml urine can be reached. |
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Fluoroquinolone, ELISA Kit |
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PN 52250B, Microtiter Plate (96T) [info...] [user guide...] [flow chart...] |
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Gentamicin, ELISA kit |
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PN 5111G, Microtiter Plate (96T)
| The Gentamicin EIA is a competitive enzyme immunoassay for the screening of a.o. milk and tissue samples on the presence of this broad spectrum antibiotic. The dose-response curve shows a measuring range of 0.1-10 ng/ml or 5 to 500 pg/well. The Gentamicin EIA shows a 25% cross-reactivity towards Sisomycin. The EIA shows no cross-reactivity (< 0.1%) towards other aminoglycosides (Neomycin, Kanamycin, Streptomycin, Dihydrostreptomycin, Tobramycin and Amikazin). Defatted and diluted milk can be applied directly into the test with a detection limit of approximately 2 ng/ml. |
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Ivermectin, ELISA kit |
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PN 51411, Microtiter Plate (96T)
| Ivermectin is an exceptionally potent drug which exhibits a broad spectrum activity against parasites in many species. This microtiter plate based EIA kit is used for the sreening of Ivermectin in tissue and milk samples. The limit of detection is 5 ng/ml. Respective cross-reactivities are: Ivermectine 100%, doramectin 13.5%, eprinomectin 16.8% and abomectin 13.3%. MRL for Ivermectin varies from 15-100 ng/kg depending on species. |
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Malachite Green, ELISA Kit |
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PN 52251B, Microtiter Plate (96T) [info...] [user guide...] |
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Neomycin, ELISA kit |
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PN 5111N, Microtiter Plate (96T)
| The Neomycin EIA is a competitive enzyme immunoassay for the screening of a.o. milk and tissue samples on the presence of this broad spectrum antibiotic. The dose-response curve shows a measuring range of 0.5-50 ng/ml or 25 to 2500 pg/well. The Neomycin EIA shows no cross-reactivity (< 0.1%) towards other aminoglycosides (Gentamicin, Kanamycin, Streptomycin, Dihydrostreptomycin, Sisomycin, Tobramycin and Amakizin). Defatted and diluted milk can be applied directly into the test with a detection limit of approximately 2 ng/ml. |
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19-Nortestosterone, ELISA kit |
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PN 5082N, Microtiter Plate (96T)
| The 19-Nortestosterone EIA is a competitive enzyme immunoassay for the screening of urine samples on the presence of 19-Nortestosterone residues like 17beta-19-Nortestosterone, 17alpha-19-Nortesterone and 19-Norandrostenedione. The dose-response curve shows a measuring range for 17beta-19-Nortestosterone of 0.05-2.0 ng/ml or 2.5-100 pg/ well. Large amounts of urine samples can be screened in a short time for the presence of 17beta-19-Nortestosterone and its major metabolites. To reduce the influence of other urine compounds a column clean up (C-18 Bakerbond) is recommended. Depending on sample preparation procedure a sensitivity down to 0.5 ng/ml can be reached. |
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Progesterone, ELISA Kit |
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PN 5081P, Microtiter Plate (96T)
| Progesterone from the ovarian corpus luteum plays a key physiological role in animal reproduction. Knowledge of progesterone level can provide valuable information for reproductive management of dairy cattle. The dose-response curve of the Progesterone-EIA shows a measuring range of 0.05 - 10 ng/ml and milk samples can be analysed directly for the concentration of progesterone at the relevant levels between 0.1 - 10 ng/ml. |
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Ractopamine, ELISA Kit |
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PN 515555, Microtiter Plate (96T) [user guide...] [flow chart...] |
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Sulfamethazine, ELISA kit |
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PN 515001, Magnetic Particle (100T) [info...] [user guide...] [flow chart...]
[news...] [publication]
| A highly sesnitive magnetic particle based ELISA that can be used as a quantitative, semi-quantitative or qualitative assay for the detection or measurement of Sulfamethazine in various sample matrixes (please refer to package insert for details). |
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PN 5101SM, Microtiter Plate (96T)
| The Sulfamethazine (Sulfadimidine) EIA is a competitive enzyme immunoassay for the screening of urine, tissue, plasma and milk samples on the presence of this antimicrobial. The dose-response curve shows a measuring range for sulfamethazine of 0.2 - 10 ng/ml. The antibody reacts with sulfamerazine (13% cross-reactivity) and shows high cross-reactivity (130%) towards the major metabolite N4-acetyl-sulfamethazine. The cross-reactivities of ten other sulfonamides (sulfadiazine, sulfadimethoxine, sulfadoxine, sulfaguanidine, sulfamethoxazole, sulfamethoxydiazine, sulfapyridine, sulfaquinoxaline, sulfathiazole and sulfatroxazole) are < 0.5%. Urine and plasma samples can be used to predict the level of sulfamethazine in tissues. In pig urine, acetylation is the major elimination pathway of sulfamethazine and about 75% is excreted as N4-acetyl-sulfamethazine. Due to the presence of the metabolite in urine and the sensitivity of the Sulfamethazine EIA, low concentrations in tissue can be screened for. Diluted urine, plasma and defatted milk samples can be analysed directly. For tissues, an extraction procedure is described. |
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Sulfadiazine, ELISA kit
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PN 5101S, Microtiter Plate (96T)
| The Sulfadiazine EIA is a competitive enzyme immunoassay for the screening of urine, tissue, plasma and milk on the presence of this antimicrobial agent. The dose-response curve of the Sulfadiazine shows a measuring range of 0.2 - 10 ng/ml. The antibodies show high cross-reactivities towards sulfamerazine (75%) and the major metabolite N4-acetyl-sulfadiazine. Other sulfonamides show low cross-reactivities: sulfathiazole (3%), sulfa-methazine (0.5%), sulfapyrine (0.5%), sulfamethoxy-diazine (0.5%), sulfamethoxine (< 0.1%), sulfadoxine (< 0.1%), sulfaguanidine (< 0.1%), sulfamethoxazole (< 0.1%), sulfaquinoxaline (< 0.1%). Sample treatment is similar to the Sulfamethazine-EIA. |
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Sulfamethoxazole, ELISA Kit |
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PN 522003, Microtiter Plate (96T) [info...] [user guide...] [poster] [news...] |
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Streptomycin, ELISA kit |
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PN 5111S, Microtiter Plate (96T)
| The Streptomycin EIA is a competitive enzyme immunoassay for the screening of milk, tissue, fat and honey samples. The dose-response curve shows a measuring range of 0.1 - 10 ng/ml or 5 to 500 pg/well. The antibodies show high cross-reactivity towards the major metabolite Dihydrostreptomycin (100%). The cross-reactivities towards other aminoglycosides (Gentamicin, Neomycin, Kanamycin, Sisomycin, Tobranycin and Amikazin) are < 0.1%. Defatted and diluted milk samples can be applied directly into the test with a detection limit for Streptomycin and Dihydrostreptomycin of approximately 2 ng/ml. |
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Trenbolone, ELISA kit |
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PN 5081T, Microtiter Plate (96T)
| Trenbolone is a xenobiotic anabolic compound with androgenic growth promoting activities which is prohibited from use in the EC member states. Following cross-reactivities were demonstrated for this Trenbolone EIA: 17-beta-Trenbolone (100%), 17-alpha-Trenbolone (63%), Altrenogest (38%), 19- Nortestosterone (2%). Endogenous androgen had less than 0.01 % cross-reactivity. In urine samples, Trenbolone can be demonstrated up to a sensitivity of 0.5 ng/ml, after a simple extraction method. |
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Tylosin, ELISA Kit |
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PN 52256B, Microtiter Plate (96T) [info...] |
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This test is based on a mixture of antibodies raised against salbutamol and clenbuterol respectively. This "generic" test detects, due to the antibody mixture, more ß-agonists than the Clenbuterol-EIA. The test is recommended for the detection of the following ß-agonist (% cross-reactivity): salbutamol (100%), clenbuterol (100%), bromobuterol (100%), cimbuterol (75%), mapenterol (50%), mabuterol (60%), tulobuterol (50%), clenpenterol (50%), terbutaline (40%), carbuterol (40%), and cimaterol (10%). The dose-response curve shows a measuring range for salbutamol of 0.1-5 ng/ml or 5-250 pg/well. Urine can be used directly in this test after pH adjustment or to lower background after extraction. For faeces, cattle feed, tissue, plasma, bile, hair and choroid/retina samples, simple extraction methods are described in the users guide. The sample preparation procedures include an enzymatic hydrolysis to guarantee valid measurements of (glucorinated/sulphated) ß-agonists like salbutamol, terbutaline and carbuterol. Detection limits, depending on the ß-agonists, sample material and clean-up procedure, down to 0.05 ng/g can be reached. |
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Zeralenone, ELISA kit |
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PN 5121Z, Microtiter Plate (96T)
| Zearalenone is an oestrogenic compound, also known as F-2 mycotoxin, which occurs in cereals and grains but also in banana's, bean leaves, etc. The antibody used in this test cross-reacts with Zeranol and its main metabolites. However, these anabolic compounds do not occur in grains, cereals, etc. The Zearalenone EIA can be used in combination with a fast and simple extraction procedure, or after a solid phase extraction method. |
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Zeranol, ELISA kit |
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PN 5081Z, Microtiter Plate (96T)
| Zeranol is a xenobiotic anabolic agent, which is legally used in several countries. However, in the EC the anabolics are banned since 1986. Because of close structural resemblance the antibody cross-reacts with both Zeranol and and the mycotoxine Zearalenone and their main metabolites. In cattle (veal calves) the occurrence of zearalenone is rare if well defined feed is administered (milk replacers and soy proteins). The Zeranol EIA is recommended for the screening of urine samples on the presence of Zeranol (100%), Taleranol (133%), Zearalanone (100%), Alpha-Zearalenol (90%), Zearalenone (75%) and Beta-Zearalenol (70%). Stilbenes show a cross-reactivity of less than 0.01% (Diethylstilbestrol, Hexestrol and Dienestrol). 17Beta-Oestradiol, Progesterone and Testosterone cross-react < 0.01%. With this Zeranol EIA a sensitivity of up to 0.1 ng/ml urine can be obtained. |
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