A group of 6th and 7th graders at Orcas Island High School in San Juan County, WA conducted a science project earlier this year looking at triclosan and 17-beta-estradiol in three local waste water treatment plants. Their report is available upon request.
The Abraxis Caffeine ELISA kit user's guide now includes applications to measure caffeine in biological fluids. Because of its very good sensitivity, discrimination between coffee drinkers, casual drinkers, and non-drinkers is possible.
With the 8 additional kits, a total of 45 of Abraxis' ELISA kits will be listed in the National Environmental Methods Index (NEMI), http://www.nemi.gov , an online, user-friendly, searchable database of method summaries funded by the U.S. Geological Survey (USGS) and the U.S. EPA. A Greenness Profile is also available for the listed kits. The Abraxis methods involve water analysis for estrogens, cyanotoxins, pesticides, and veterinary residues. Abraxis is committed to adding more analytes as they become available.
Abraxis has demonstrated the performance of their microtiter plate format Microcystins/Nodularins (ADDA) and DM ELISA kits as well as the lateral flow, dipstick, Recreational Water Strip Test Kit by participating in the ETV Program.
The U.S. Environmental Protection Agency (EPA) has established the Environmental Technology Verification (ETV) Program to facilitate the deployment of innovative or improved environmental technologies through performance verification and dissemination of information. The goal of the ETV program is to further environmental protection by accelerating the acceptance and use of improved and cost-effective technologies. ETV seeks to achieve this goal by providing the high-quality, peer-reviewed data on technology performance to those involved in the design, distribution, financing, permitting, purchase, and use of environmental technologies.
ETV works in partnership with recognized standards and testing organizations, with stakeholder groups and with individual technology developers. The program evaluates the performance of innovative technologies by developing test plans that are responsive to the needs of stakeholders, conducting field or laboratory tests, collecting and analyzing data, and preparing peer-reviewed reports. All evaluations are conducted in accordance with rigorous quality assurance (QA) protocols to ensure that data of known and adequate quality are generated and that the results are defensible.
These three kits join the previously verified kits, Atrazine, 17-Beta Estradiol, and OP/C, in proudly displaying the EPA's ETV trademark on their product literature and packaging.
The Abraxis Cotinine ELISA kit user's guide now includes applications to measure cotinine in biological fluids. Because of its very good sensitivity, discrimination between smokers, passive smokers, and non-smokers is possible.
Both the Abraxis' Cylindrospermopsin and Saxitoxin ELISA kits are referenced in the Standardized Analytical Methods (SAM) for Environmental Restoration Following Homeland Security Events, EPA/600/R-04/126D, 29 Sep. '08.
Abraxis introduced the world's first quantitative, microtiter plate (96T) format ELISA kit for microcystins and nodularins in human serum samples.
Abraxis introduced an Acrylamide ELISA kit at SNAXPO 2010 in Fort Worth, TX, March 4th & 5th. The kit, PN 515675, uses a microtiter plate format with 12 removable strips of 8 wells (96T) and provides a real-time, on-site, economical, quantitative, accurate, sensitive, analytical alternative for risk management of Acrylamide in both snacks and fast foods.
Acrylamide is found as a natural byproduct of the cooking process. Methods in which temperature exceeds 120°C, such as bakin, frying, grilling, and toasting can cause the amino acid asparagine to react with reducing sugars such as glucose to produce Acrylamide. High carbohydrate foods that are baked or fried at high temperatures contain the highest levels of Acrylamide.
Acrylamide is an essential industrial chemical with an estimated worldwide production of 200 million kg/year. It is used as a grouting agent in construction, a paper making aid, a soil conditioning agent in ore processing, in sewage treatment, and as a coagulant in water treatment. Acrylamide is a known carcinogen in laboratory animals, impairing fertility in male animals and causing nerve damage in humans with industrial exposure.
A recently published study in The Journal of Dental Hygiene used the Abraxis BPA ELISA Kit to detect and quantitate levels of BPA in saliva and serum prior to and after placement of dental sealants in adults. The direct competitive ELISA protocol was used on the recognition of BPA by specific monoclonal antibodies. Distinctively, the BPA ELISA used in this study provided a high level of sensitivity, with a detection range from 0.05 µg/L to 10 µg/L and coefficient of variation less than 10%.
Abraxis now provides both a (homo)anatoxin-a and a deoxycylindrospermopsin standard for cyanotoxin testing by traditional analytical methods. Both standards are at a concentration of 10 µg/mL.
"As you may recall, last July my 11 year-old daughter used your sulfamethanzine ELISA magnetic particle test kit (with photometer and related test equipment) to perform an experiment removing sulfamethazine from contaminated groundwater. I'm pleased to report that at last week's Florida State Science Fair she received the Best-in-State nod from the judges: (a) First Place, Environmental Science, Junior Division; and (b) Best in Fair, Physical Sciences, Junior Division (i. e. the Grand Prize Award). The Florida State Science and Engineering Fair was a competition between the top 829 science fair projects in Florida form an original pool of more than 100,000 science fair projects at the local school level. My daughter advance to the State Competition as the First Place award winner in Environmental Science from the Palm Beach County Regional Science and Engineering Fair.
My daughter and I thank you for your past support and for producing a superlative ELISA test that can be both understood and expertly performed by a middle school student. My daughter told me that she explained the Abraxis ELISA test multiple times to numerous judges and answered all of their questions about your product. She understands the technology, procedures, and why the test works. Bravo!
Thanks again for all of your past support and help. We are delighted to continue our relationship with Abraxis! Thank you Curtis and thank you Abraxis for helping to produce a Best-in-State science fair project."
Four ELISA kits are now available for the determination of Lasalocid, Maduramicin, Monensin, and Salinomycin/Narasin. Developed for low level detection with a LOQ of 30 ppb, these kits are ideal for testing feeds for avians and mammals, such as cats and dogs, suseptable to very low levels of ionophore toxicity. The kits use a common, greatly simplified sample clean up allowing for real time results.
Abraxis introduced an ELISA kit for the controversial cyanotoxin BMAA at the 8th National Monitoring Conference in Portland, OR, April 30th - May 4th. The kit, PN 520040, uses a microtiter plate format with 12 removable strips of 8 wells (96T). Incorporated into the kit is new technology for which a patent has been applied for. The kit provides a real-time, economical, quantitative, accurate, sensitive, analytical alternative for both research and monitoring programs.
AWWA recommends using the List 3 component of UCMR3 to do targeted monitoring of anatoxin-a, microcystin-LR, and/or cylindrospermopsin. Given available methods and laboratory capacity, developing a Pre-Screening Testing Survey for algal toxins would be more likely to collect useful data than the proposed ground water monitoring using qPCR testing. AWWA recommends EPA develop a special sampling plan for algal toxins using ELISA assays, backed up by research-grade LC-MS-MS methods. This monitoring should not be based on a routine quarterly or semi-annual schedule, but rather include samples collected based on the occurrence of blooms and limited to utilities that have surface water supplies with appropriate bloom formation conditions.
Please click on the link below for further infomation:
In response to a recent study conducted by a German university that found very high concentrations of glyphosate in human urine samples, we now offer an application for this matrix. The amount of glyphosate found in the urine by this study was elevated 5 to 20-fold the limit established for drinking water.
Removal of Sulfamethazine by Hypercrosslinked Adsorbents in Aquatic Systems.
J. Environ. Qual. 42:2-9 (2013).
... is the title of a report authored by Morgan Basiuk and Rachel Brown of the South East Alberta Water Alliance (seawa). Through the cooperative efforts of the Alberta Association of Colleges and Technical Institutes, the City of Medicine Hat, Hyperion Research Ltd., a private donation and the Defense Research and Development Canada, the work, using thirteen Abraxis ELISA kits, was performed during the summer of 2012.
"What is in Our Waterways?--- AN OVERVIEW
Pharmaceuticals, personal care products, industrial chemicals and endocrine disruptors in the water-cycle are becoming increasingly concerning. In the last decade, traces of pharmaceuticals in the levels of nanograms to micrograms per liter have been reported in surface waters, wastewater, groundwater and, to a smaller degree, drinking water. These chemicals can enter the environment and waterways from human and animal excretion, wastewater effluent, treated sewage sludge, industrial waste, medical waste and landfill leachate.
It is important to determine the presence of these chemicals in the waterways to determine the necessity to implement policies and remediation procedures. The presence of these chemicals in the environment is concerning for a number of reasons. One major concern is the continual increase in the development of resistance to pharmaceuticals and antimicrobial products. Furthermore, the accumulation and presence of these chemicals in the water ways can affect animal and human health in a multitude of ways. To a great extent however, these harmful health effects are unknown due to the lack of research. What is known is that many of these compounds have cancer-causing effects and some can act as endocrine-disrupters by mimicking the body’s hormones. Thus, this research is very important due to the potential these chemicals have to harm the aquatic environment and subsequently humans if they make their way into drinking water.
This particular study was conducted to evaluate the methodology for the determination of several chemicals in water samples using Enzyme-Linked Immunosorbent Assay (ELISA) kits purchased from Abraxis LLC (Warminster, PA, USA). Methodological protocols were adapted from the procedures provided by Abraxis LLC and trials were conducted. Provided kit standards of known chemical concentrations were put through multiple trials until confidence was built in the methodology and consistent linear standard curves were produced. A methodology for appropriate analysis of the resulting absorbance values was also determined. Precise and accurate methods were determined for each compound that we now practice with confidence.
A secondary objective of this research was to determine the concentrations of these chemicals at selected sites around Medicine Hat. These included the South Saskatchewan River downstream of Medicine Hat, Seven Persons Creek, Ross Creek, Medicine Hat Wastewater Treatment Plant influent and effluent, tap water and deionized water. The following chemicals were analyzed: alkylphenols, sulfamethazine, sulfamethoxazole, glyphosates, benzo(a)pyrene, triclosan, estrogens, ethinylestradiol, testosterone, avermectins, 2,4-D, microcystins and carbamates. The results for each of these compounds are outlined in separate reports for each compound. These reports include background information on the chemical, the methodology we determined and tabulated data of the resulting concentrations."
A copy of the report is available upon request.
Abraxis announces the commercialization and immediate availability of both its Immunomagnetic Separation (IMS) Beads for the "Big Six" non-O157 shiga toxin production E. coli (STEC) as well as the expansion of its LAT product line to now include a kit for O104.
The IMS products are intended for the rapid and selective concentration of non-O157 STEC serogroups O26, O45, O103, O111, O121, and O145 as described in the USDA-FSIS Microbiological Laboratory Guidebook (MLG) 5B.02.
The O104 LAT kit is intended for the identification of presumptive positive colonies and complements the existing six kits which are based on the work of Medina, M. et. al. entitled "Latex Agglutination Assays for Detection of Non-O157 Shiga Toxin-Producing Escherichia coli Serogroups O26, O45, O103, O111, O121, and O145" published in the Journal of Food Protection 75(5): 819-826 and also presented at the annual meeting of the International Association for Food Protection (IAFP).
The Abraxis Latex Agglutination Test (LAT) kits for the "Big Six" non-O157 shiga toxin producing E. coli (STEC) have been reviewed by the
be used in non-O157 STEC testing.
The kits are intended for the identification of presumptive positive
colonies as described in the USDA MLG and are based on the work of Medina,
M. et. al. entitled "Latex Agglutination Assays for Detection of Non-O157
Shiga Toxin - Producing Escherichia coli Serogroups O26, O45, O103, O111,
O121, and O145" published in the Journal of Food Protection 75(5):819-826
and also presented at the recent annual meeting of the International
Association for Food Protection (IAFP).
A recently published paper in the journal Environmental Science and
Technology correlated urbanization with a rise in triclosan concentrations
in sediments and the proportion of bottom-dwelling bacteria resistant to
triclosan. Combined sewer overflows that release untreated sewage are a
major source of triclosan pollution. There are nearly 800 cities in the US
that use combined sewers. Triclosan exposure triggers an increase in
triclosan-resistent bacteria. Futhermore, there is a decrease in the
diversity of benthic bacteria and a shift in the composition of bacterial
communities. Most notable were a 6-fold increase in cyanobacteria and a
dramatic die-off of algae.
Abraxis provides kits for monitoring triclosan and cyanotoxins.
Azoxystrobin and pyraclostrobin are among nine fungicides recently reviewed in a USGS report for their effects on fish and invertabrates in the aquatic environment. They were identified as emerging chemicals of concern because of their high or increasing global use rates, detection frequency in surface water, or likely persistence in the environment.
Domestic historical data shows that 91% of the total pounds applied of azoxystrobin for agricultural use were with rice (56%), peanuts (24%) and grapes (11%). More recently, another USGS report shows azoxystrobin was detected in 45% of the targeted samples and that selected application of`fungicides has grown substantially over the past decade.
The two kits will be submitted for inclusion in the National Methods Index (NEMI), http://www.nemi.gov, an online, user-friendly, searchable data base of method summaries that is funded by the USGS and US EPA. With the two additions, a total of 47 of Abraxis's kits will be listed in NEMI.
Abraxis introduces a patented, microtiter plate format (96T),
receptor-binding assay (RBA) for the Anatoxin-a. Practioners can select
between two protocols. Both protocols require incubations at 37C. The "EZ"
protocol requires no sample preparation and has a range of 5 to 500 ppb. An
enhanced sensitivity (ES) SPE sample concentration may be performed for
programs requiring a lower limit of detection. The kit provides a
real-time, economical, quantitative, accurate, sensitive, analytical
alternative for both research and monitoring programs.
Anatoxin-a is an alkaloid neurotoxin produced by some species of
cyanobacteria. It is one of the most toxic of the cyanotoxins. In humans
and other animals, the skeletal neuromuscular junction constitutes a primary
target for Anatoxin-a. Anatoxin-a can also cross the blood-brain barrier.
The neuromuscular junction is specialized for the rapid transmission of neuronal information from the pre-synaptic nerve
terminal to the post-synaptic muscle fiber. This transmission is mediated by
the synchronous release of the neurotransmitter acetylcholine (ACh), which
activates nicotinic acetylcholine receptors (nAChRs) in the muscle endplate,
triggering a series of events that lead to muscle contraction. Most ACh
molecules are hydrolyzed by acetylcholinesterases, which are highly
concentrated at the neuromuscular junction. Anatoxin-a functions as an
agonist of nAChRs, like ACh, but is about 20 times more potent. Unlike ACh,
it is not degraded by acetylcholinesterases and produces sustained depolarization of the muscle
endplate, causing overstimulation of the muscles, leading to muscle fatigue
and ultimately paralysis. Symptoms begin within 5 minutes of ingestion of
Anatoxin-a and progress rapidly, resulting in cyanosis, convulsions, cardiac
arrhythmia, and respiratory paralysis, which ultimately results in death due
to suffocation. Humans and other animals may be exposed to Anatoxin-a
through ingestion of contaminated water via drinking or during recreational
activities in which water is swallowed. Due to the potential for serious
harm and even death, many countries are expanding monitoring programs to
include Anatoxin-a and are establishing regulations regarding the amount of
Anatoxin-a in drinking and recreational waters.
User's guide and flow chart for the "EZ" protocol are posted to our web
site. Pricing and the ES sample concentration technical bulletin are
available upon request.
The death of a dozen racing horses and another 18 that have fallen ill has exposed a western hemisphere feed manufacture to a multimillion dollar liability. Laboratory analyses found narasin, an ionophore, in 80% of the samples tested. Narasin is often added to broiler chicken feeds for prevention of coccidiosis, a disease of birds and mammals that chiefly affects the intestines. Ingestion of narasin by horses, cattle, turkeys, dogs, cats, rabbits and rats has proven to be fatal.
The risks of ionophore contaminated feed producing deaths and illnesses are minimized with an inexpensive, on site, real time testing system.
Microcystins Dipsticks Helps Promote Safety of Participants at Triathlon Competition